Sample Course Schedule
CRISPR/Cas9 Genome Editing: A Hands-on Experience
|
Time |
Wednesday |
Thursday |
Friday |
Saturday |
|---|---|---|---|---|
|
7:00 |
Breakfast 7 - 8:30 am |
Breakfast 7 - 8:00 am |
Breakfast 7 - 8:00 am |
|
|
7:30 |
||||
|
8:00 |
||||
|
8:30 |
IT Session - Meigs: Access to network and shared drives |
Check embryos |
Check embryos |
|
|
9:00 |
|
Lab - Loeb 207: Injections of gRNAs and dyes, |
Lab - Loeb 207: Injections, Score Phenotypes, dechorinate, fix and do phalloidin and dapi stain |
Lab - Loeb 207: score second round of phenotypes, wrap up discussion about phenotypes and CRISPR, discussion on ethics and CRISPR. Archiv's tour and Gift shop time |
|
9:30 |
|
|||
|
10:00 |
Arrival & Check-In: 10:00 am - 12:00 pm |
|||
|
10:30 |
||||
|
11:00 |
||||
|
11:30 |
Tour - MRC tank room |
|||
|
12:00 |
Lunch Noon - 1:00 |
Lunch - Noon to 1:30 pm |
Lunch - Noon to 1:30 pm |
Lunch - Noon to 1:30 pm |
|
12:30 |
||||
|
1:00 |
MBL Orientation & Safety Training |
|||
|
1:30 |
Lab - Loeb 207: Intro to Imaging competition. Intro to fluorescent imaging, GFP Lines, Fluorescent Staining, maybe hair cell staining & Time Lapse Development |
Lab - Loeb 207: Image fixed, stained animals |
Departure & Check-Out: 1:30 pm |
|
|
2:00 |
Lecture - Loeb 263: Introduction to embryology & development & overview of how CRISPR works |
|||
|
2:30 |
||||
|
3:00 |
Lab - Loeb 207: Introduction to Microscopes and learn how to inject |
|||
|
3:30 |
||||
|
4:00 |
||||
|
4:30 |
||||
|
5:00 |
Dinner 5:30 to 6:30 pm |
Pizza & Movie Night beginning at 6 pm, Meigs Room, Swope Center |
Dinner 5:30 to 6:30 pm |
|
|
5:30 |
||||
|
6:00 |
||||
|
6:30 |
|
Work on images for image competition, submit by 10pm |
||
|
7:00 |
||||
|
7:30 |